TRABAJOS DE INVESTIGACION

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    De novo RNA-Seq analysis of the oleauginous microalgae Ankistrodesmus sp. UCP0001: Gene identification and metabolic pathways reconstruction for the biosynthesis of fatty acids and triacylglycerols
    (Plant Cell Biotechnology and Molecular Biology 18(5-6): 219-230, 2017-05-15) Castro, Juan C.; Maddox, J. Dylan; Paredes, Jae D.; Rodríguez, Hicler N.; Aguilar, Carla P.; Marapara, Jorge L.; Castro, Carlos G.; Casuso, María Z.; Cobos, Marianela
    Microalgae have great potential as feedstock to produce next-generation biofuels. The scarceness of genomic level information, however, prevents the rational de novo microalgae strain design. In this research, we describe the next-generation sequencing, de novo assembly, and functional annotation of the transcriptome of Ankistrodesmus sp. UCP0001. In total 48,867,830 high-quality sequence reads were de novo assembled into 38,414 unigenes (mean length = 508 bp, N50 = 1038 bp). Seventy-two percent of unigenes presented mapping information. Based on the KEGG pathway assignment, the fatty acids and the triacylglycerol biosynthesis pathways were reconstructed. Our results demonstrate that the synergy among high-throughput sequencing technologies and appropriate bioinformatics tools provides a fast, low-cost, and effective approach to generate invaluable functional genomic information in non-model microalgae species (e.g., Ankistrodesmus sp.). With the de novo assembled and annotated transcriptome we have successfully identified genes encoding enzymes and reconstructed metabolic pathways for the biosynthesis of fatty acids and triacylglycerols in this microalgae species. This genetic information could be used for the de novo microalgae strain design with desirable characteristics to produce biodiesel and capabilities for the biosynthesis of others valuable bioactive compounds of interest to the pharmacological, food, and cosmetic industries.
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    Media compositions affects the fatty acids profiles of three oleaginous microalgae from peruvian amazon
    (Asian Journal of Microbiology and Biotechnology, 1(1), 14-24, 2017-01-21) Cobos, Marianela; Lopez, Liz C.; Paredes, Jae D.; Maddox, J. Dylan; Vargas-Arana, Gabriel E.; Flores, Leenin; Aguilar, Carla P.; Castro, Juan C.
    The objective of this research was to determine the effect of four culture media on the fatty acids profile of the microalgae Ankistrodesmus sp., Chlorella sp., and Scenedesmus sp. isolated from the Peruvian Amazon. The microalgae species were growth by triplicate in Beijerinck's medium (BM), Blue Green medium (BG-11), Bold Basal medium (BBM), and CHU-10 medium. Growth was monitored by spectrophotometric analysis at 680 nm. Biomass productivity, biochemical analyzes, and fatty acids profiling were performed using standard methods. The highest growth was obtained with BM, BBM, and CHU-10 medium for the three microalgae species and the biomass productivity was highest (> 0.23 mg.L-1.d-1) in CHU-10 medium. The highest total lipid content was reported with Ankistrodesmus sp. (13.77 - 21.58%). Carbohydrate content varied between 8.46 and 50.17%, with the highest percentage when the microalgae were cultured in BG-11 (27.26 – 37.80%) and CHU-10 media (30.72 – 50.17%). Also, the highest protein content was found in the three microalgae species cultured in BM medium (24.89 - 30.44%). The fatty acids profiles showed differences at the intra-species and inter-species levels. In conclusion, the three microalgae showed differences in their growth profile, biomass productivity, biochemical composition (carbohydrates, total lipids, and proteins contents), and fatty acids profiles. Likewise, these growth and biochemical parameters are markedly affected by the media composition. Additionally, the fatty acids profiles of the three microalgae reveals that are a good feedstocks for biodiesel production.