Prevalencia de malaria en la avifauna del centro de investigación concesión de conservación cuenca alta río Itaya – cccari
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2022-04-13
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Universidad Científica del Perú
Abstract
Las investigaciones que se realizan sobre malaria en aves son de suma importancia, porque nos proporcionan información valiosa sobre los niveles de salud en los ecosistemas del planeta. La región amazónica de nuestro país se caracteriza por albergar una gran cantidad de especies de aves, sin embargo, también presenta la más alta tasa de deforestación, lo cual se conoce que contribuye a la aparición y aceleramiento de contagio de enfermedades en la avifauna. Por tal motivo la presente investigación tuvo como objetivo; Determinar la prevalencia de malaria en la avifauna del Centro de Investigación Concesión de Conservación Cuenca Alta Rio Itaya – CCCARI. Se utilizó redes de neblina para capturar las aves, mediante punción braquial se extrajo muestras de sangre los cuales fueron transportados al Laboratorio de Biotecnología y Bioenergética – UCP y mediante técnicas de extracción se obtuvo el ADN, los cuales fueron sometidos a pruebas de PCR (Reacción en Cadena de la Polimerasa). Por diferentes tiempos de amplificación y la utilización de cebadores se determinó la presencia o ausencia del parasito de la malaria en las aves. Se logró capturar 281 individuos de aves, pertenecientes a 66 especies y 18 familias, de los cuales el 60,2 %, de un total de 99,4 % de las familias de aves registradas, dieron resultados positivos para malaria. Se concluye que se logró identificar la mayor cantidad de la diversidad de especies que habitan los bosques de la cuenca media alta del río Itaya. Así mismo la especie que predomina estos bosques es Lepidothrix coronata y Glyphorynchus spirurus, el cual está asociado principalmente al tipo bosques que existe en la zona.
Research on malaria in birds is of the utmost importance, because it provides us with valuable information on health levels in the ecosystems of the planet. The Amazon region of our country is characterized by a large number of bird species; however, it also has the highest rate of deforestation, which is known to contribute to the emergence and acceleration of disease transmission in poultry. For this reason, the present research had as objective; To determine the prevalence of malaria in the avifauna of the Research Center Conservation Concession Upper Itaya River Basin - CCCARI. Fog nets were used to capture birds, blood samples were extracted by brachial puncture and transported to the Laboratory of Biotechnology and Bioenergetics - UCP and by extraction techniques the DNA was obtained, which were tested for PCR (Polymerase Chain Reaction). For different amplification times and the use of primers, the presence or absence of malaria parasite in birds was determined. It was possible to capture 281 birds belonging to 66 species and 18 families, of which 60.2% out of a total of 99.4% of the registered bird families tested positive for malaria. It is concluded that the greatest amount of the diversity of species that inhabit the forests of the upper middle basin of the Itaya River was identified. Likewise, the species that predominates these forests is Lepidothrix coronata and Glyphorynchus spirurus, which is mainly associated with the type forests that exist in the area.
Research on malaria in birds is of the utmost importance, because it provides us with valuable information on health levels in the ecosystems of the planet. The Amazon region of our country is characterized by a large number of bird species; however, it also has the highest rate of deforestation, which is known to contribute to the emergence and acceleration of disease transmission in poultry. For this reason, the present research had as objective; To determine the prevalence of malaria in the avifauna of the Research Center Conservation Concession Upper Itaya River Basin - CCCARI. Fog nets were used to capture birds, blood samples were extracted by brachial puncture and transported to the Laboratory of Biotechnology and Bioenergetics - UCP and by extraction techniques the DNA was obtained, which were tested for PCR (Polymerase Chain Reaction). For different amplification times and the use of primers, the presence or absence of malaria parasite in birds was determined. It was possible to capture 281 birds belonging to 66 species and 18 families, of which 60.2% out of a total of 99.4% of the registered bird families tested positive for malaria. It is concluded that the greatest amount of the diversity of species that inhabit the forests of the upper middle basin of the Itaya River was identified. Likewise, the species that predominates these forests is Lepidothrix coronata and Glyphorynchus spirurus, which is mainly associated with the type forests that exist in the area.
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Avifauna, Prevalencia, Malaria, ADN, Avifauna, Prevalence, Specific richness, Malaria, DNA
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